Effect of miR-382 on triple negative breast cancer cell line 4T1 by targeting PGC-1?

DOI: 10.14800/rd.449

Abstract

Breast cancer is one of the most frequent and malignant types of cancer in women, with an increasing morbidity and mortality rate. Treatment of triple negative breast cancer remains a challenge, since the efforts made with targeted therapies were ineffective. MicroRNAs (miRNAs) could play important roles in cancers via post-transcriptionally regulating target genes via binding to specific sequences in the 3'-UTR of downstream target genes. In the present study, the effect of miR-382 on the biological behaviors of triple negative breast cancer 4T1 cells is investigated. The expression of PGC-1? in the 4T1 cells transfected with miR-382 mimics was significantly decreased, while it was obviously increased after transfection with anti-miR-382. Moreover, the cell migration and proliferation activity were significantly increased in the miR-382+pCDNA-PGC-1? group when compared with the control+pCDNA3.1 group. However, the antitumor effect of miR-382 was blocked by overexpression of PGC-1?. Our results showed that miR-382 inhibits the proliferation and metastasis of 4T1 cells by down-regulating PGC-1?, suggesting miR-382 might be a therapeutic target in triple negative breast cancer.