Expression of ITGB1 and E-cadherin regulated by lncLSINCT5 sponging on miR-29c-3p in papillary thyroid cancer cells

DOI: 10.14800/rd.451

Abstract

Papillary thyroid carcinoma (PTC) is the most predominant subtype of thyroid cancer, contributing to more than 80% of all thyroid or endocrine malignancies. However, the role of Long noncoding RNA long stress induced non-coding transcripts (LSINCT5) in papillary thyroid cancer remains largely unknown. In the present study, we found that the expression of LSINCT5 in PTC cell line was higher than that in human normal thyroid cell HT-ori3. The proliferation and migration ability of PTC cell lines TPC-1 and KAT-5 cells were significantly decreased after transfection of siLSINCT5-1 and siLSINCT5-2 compared with siNC transfection. The dual luciferase reporter gene and RIP confirmed that LSINCT5 is capable of specifically binding to miR-29c. Compared with the transfected LSINCT5 group, the proliferation and migration ability of TPC-1 cells in the co-transfected LSINCT5 and miR-29c groups were significantly decreased, and the expression of ITGB1 mRNA and protein were down-regulated either. Taken together, our data indicated that LSINCT5 can inhibit the tumor suppressive effect of miR-29c-ITGB1 axis and promote the proliferation and metastasis of PTC by targeting miR-29c-ITGB1 axis.